Review



rabbit anti human cd86  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 95
      Buy from Supplier

    Structured Review

    Proteintech rabbit anti human cd86
    Expression of CD68, <t>CD86</t> and CD206 in decidual tissues of normal pregnancy women and patients with PE. ( A ) The expression levels of CD68, CD86 and CD206 in decidual tissues were assessed by immunohistochemistry (×200). ( B ) Semiquantitative analysis of ( A ). ( C ) The relative protein levels of CD86 and CD206 were detected via Western blotting. GAPDH was used as an endogenous control. ( D ) Semiquantitative analysis of ( C ). ( E ) The relative mRNA levels of TNF-α and IL-6 were detected via q-PCR. Student’s t -test was used for the statistical analysis, and *p<0.05; **p < 0.01.
    Rabbit Anti Human Cd86, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+cd86/pmc12714541-63-32-36?v=Proteintech
    Average 95 stars, based on 31 article reviews
    rabbit anti human cd86 - by Bioz Stars, 2026-08
    95/100 stars

    Images

    1) Product Images from "Altered Expression of CXCL16/CXCR6 and Its Correlation with Decidual Macrophage Polarization in Preeclampsia"

    Article Title: Altered Expression of CXCL16/CXCR6 and Its Correlation with Decidual Macrophage Polarization in Preeclampsia

    Journal: International Journal of Women's Health

    doi: 10.2147/IJWH.S567928

    Expression of CD68, CD86 and CD206 in decidual tissues of normal pregnancy women and patients with PE. ( A ) The expression levels of CD68, CD86 and CD206 in decidual tissues were assessed by immunohistochemistry (×200). ( B ) Semiquantitative analysis of ( A ). ( C ) The relative protein levels of CD86 and CD206 were detected via Western blotting. GAPDH was used as an endogenous control. ( D ) Semiquantitative analysis of ( C ). ( E ) The relative mRNA levels of TNF-α and IL-6 were detected via q-PCR. Student’s t -test was used for the statistical analysis, and *p<0.05; **p < 0.01.
    Figure Legend Snippet: Expression of CD68, CD86 and CD206 in decidual tissues of normal pregnancy women and patients with PE. ( A ) The expression levels of CD68, CD86 and CD206 in decidual tissues were assessed by immunohistochemistry (×200). ( B ) Semiquantitative analysis of ( A ). ( C ) The relative protein levels of CD86 and CD206 were detected via Western blotting. GAPDH was used as an endogenous control. ( D ) Semiquantitative analysis of ( C ). ( E ) The relative mRNA levels of TNF-α and IL-6 were detected via q-PCR. Student’s t -test was used for the statistical analysis, and *p<0.05; **p < 0.01.

    Techniques Used: Expressing, Immunohistochemistry, Western Blot, Control

    Immunofluorescence staining for M1 and M2 in normal pregnancy and patients with PE. CD68 is the maker for pan-macrophages. CD80 and CD86 are markers for M1, CD163 and CD206 are markers for M2 macrophages. Scale bars: 100 µm. ( A and B ) The CD68 + CD80 + and CD68 + CD86 + cells in decidual tissues were detected by immunofluorescence double staining. ( C and D ) The CD68 + CD163 + and CD68 + CD206 + cells in decidual tissues were detected by immunofluorescence double staining.
    Figure Legend Snippet: Immunofluorescence staining for M1 and M2 in normal pregnancy and patients with PE. CD68 is the maker for pan-macrophages. CD80 and CD86 are markers for M1, CD163 and CD206 are markers for M2 macrophages. Scale bars: 100 µm. ( A and B ) The CD68 + CD80 + and CD68 + CD86 + cells in decidual tissues were detected by immunofluorescence double staining. ( C and D ) The CD68 + CD163 + and CD68 + CD206 + cells in decidual tissues were detected by immunofluorescence double staining.

    Techniques Used: Immunofluorescence, Staining, Double Staining

    Decidual macrophages polarization of normal pregnant women and patients with PE. ( A ) Flow cytometric analysis to determine the proportion of M1 (CD68 + CD80 + or CD68 + CD86 + ) and M2 (CD68 + CD163 + or CD68 + CD206 + ) in normal pregnancy and patients with PE. ( B ) Semiquantitative analysis of ( A ). Student’s t -test was used for the statistical analysis, and **p<0.01; ***p < 0.001; ****p < 0.0001.
    Figure Legend Snippet: Decidual macrophages polarization of normal pregnant women and patients with PE. ( A ) Flow cytometric analysis to determine the proportion of M1 (CD68 + CD80 + or CD68 + CD86 + ) and M2 (CD68 + CD163 + or CD68 + CD206 + ) in normal pregnancy and patients with PE. ( B ) Semiquantitative analysis of ( A ). Student’s t -test was used for the statistical analysis, and **p<0.01; ***p < 0.001; ****p < 0.0001.

    Techniques Used:



    Similar Products

    rabbit anti human cd86  (Proteintech)
    95
    Proteintech rabbit anti human cd86
    Expression of CD68, <t>CD86</t> and CD206 in decidual tissues of normal pregnancy women and patients with PE. ( A ) The expression levels of CD68, CD86 and CD206 in decidual tissues were assessed by immunohistochemistry (×200). ( B ) Semiquantitative analysis of ( A ). ( C ) The relative protein levels of CD86 and CD206 were detected via Western blotting. GAPDH was used as an endogenous control. ( D ) Semiquantitative analysis of ( C ). ( E ) The relative mRNA levels of TNF-α and IL-6 were detected via q-PCR. Student’s t -test was used for the statistical analysis, and *p<0.05; **p < 0.01.
    Rabbit Anti Human Cd86, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+cd86/pmc12714541-63-32-36?v=Proteintech
    Average 95 stars, based on 1 article reviews
    rabbit anti human cd86 - by Bioz Stars, 2026-08
    95/100 stars
    		  Buy from Supplier
    rabbit anti human cd86  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc rabbit anti human cd86
    A Schematic overview of the experimental strategy (Created in BioRender). Pan, W. (2025) https://biorender.com/udc23v6 . Surgical tumor specimens and paired normal adjacent tissues (NATs) were collected from 23 treatment-naive PSCC patients (Tumor, n = 23; NATs, n = 5) and processed for single-cell RNA sequencing and transcriptomic analysis. B UMAP plot of 202,424 cells from the discovery cohort, showing cell components in PSCC, color-coded by cell type (left), patients (middle) and tissue (right). C Normalized expression of marker genes used to define four major cell types as shown in ( B ). D Relative abundance of four major cell types in tumor tissues ( n = 23) or NATs ( n = 5) from each PSCC patient, excluding PN4. E Bar graph comparing the relative proportions of the four cell types between tumor and NATs. P- values were assessed using a two-sided unpaired Student’s t test (Immu: **** P < 0.0001, Endo: **** P < 0.0001, CAFs: ** P = 0.002). F Multiplex immunofluorescence staining depicting the presence of four major cell components (CD45 + immune cells, KRT5 + epithelial cells, CD31 + endothelial cells, and VIM + fibroblasts) in tumor and NATs of PSCC. Representative images include DAPI + nuclei for reference. Scale: 50 μm. G Multiplex immunofluorescence depicting the distribution of five major immune subpopulations (CD4 + T cells, CD8 + T cells, <t>CD86</t> + antigen-presenting cells, CD20 + B cells, CD56 + natural killer cells) in tumor and NATs of PSCC. Representative images include DAPI + nuclei for reference. Scale: 200 μm. H Quantification of CD4 + , CD8 + , CD86 + , CD20 + and CD56 + cells per area in paired tumor and NATs regions in PSCC ( n = 6). P- values were assessed using a two-sided paired Student’s t test (CD4 + : **** P < 0.0001, CD8 + : *** P = 0.0002, CD86 + : *** P = 0.0002, CD20 + : **** P < 0.0001, CD56 + : ** P = 0.0053). I UMAP plot comparing the TME composition in PSCC with other squamous cell carcinomas, including HNSCC ( GSE103322 ), NPC ( GSE150430 ), and cSCC ( GSE144236 ). J Bar graph showing the relative proportions of the four cell types across these tumor types. PN PSCC adjacent normal tissue, PT PSCC tumor tissue. NATs Normal adjacent tissues. HNSCC: Head and neck squamous cell carcinoma, NPC Nasopharyngeal carcinoma, cSCC Cutaneous squamous cell carcinoma. Data are presented as mean ± s.e.m. ns: not significant ( P > 0.05). Source data are provided as a Source Data file.
    Rabbit Anti Human Cd86, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+cd86/pmc12816729-368-45-47?v=Cell+Signaling+Technology+Inc
    Average 96 stars, based on 1 article reviews
    rabbit anti human cd86 - by Bioz Stars, 2026-08
    96/100 stars
    		  Buy from Supplier
    human anti cd86  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc human anti cd86
    A Schematic overview of the experimental strategy (Created in BioRender). Pan, W. (2025) https://biorender.com/udc23v6 . Surgical tumor specimens and paired normal adjacent tissues (NATs) were collected from 23 treatment-naive PSCC patients (Tumor, n = 23; NATs, n = 5) and processed for single-cell RNA sequencing and transcriptomic analysis. B UMAP plot of 202,424 cells from the discovery cohort, showing cell components in PSCC, color-coded by cell type (left), patients (middle) and tissue (right). C Normalized expression of marker genes used to define four major cell types as shown in ( B ). D Relative abundance of four major cell types in tumor tissues ( n = 23) or NATs ( n = 5) from each PSCC patient, excluding PN4. E Bar graph comparing the relative proportions of the four cell types between tumor and NATs. P- values were assessed using a two-sided unpaired Student’s t test (Immu: **** P < 0.0001, Endo: **** P < 0.0001, CAFs: ** P = 0.002). F Multiplex immunofluorescence staining depicting the presence of four major cell components (CD45 + immune cells, KRT5 + epithelial cells, CD31 + endothelial cells, and VIM + fibroblasts) in tumor and NATs of PSCC. Representative images include DAPI + nuclei for reference. Scale: 50 μm. G Multiplex immunofluorescence depicting the distribution of five major immune subpopulations (CD4 + T cells, CD8 + T cells, <t>CD86</t> + antigen-presenting cells, CD20 + B cells, CD56 + natural killer cells) in tumor and NATs of PSCC. Representative images include DAPI + nuclei for reference. Scale: 200 μm. H Quantification of CD4 + , CD8 + , CD86 + , CD20 + and CD56 + cells per area in paired tumor and NATs regions in PSCC ( n = 6). P- values were assessed using a two-sided paired Student’s t test (CD4 + : **** P < 0.0001, CD8 + : *** P = 0.0002, CD86 + : *** P = 0.0002, CD20 + : **** P < 0.0001, CD56 + : ** P = 0.0053). I UMAP plot comparing the TME composition in PSCC with other squamous cell carcinomas, including HNSCC ( GSE103322 ), NPC ( GSE150430 ), and cSCC ( GSE144236 ). J Bar graph showing the relative proportions of the four cell types across these tumor types. PN PSCC adjacent normal tissue, PT PSCC tumor tissue. NATs Normal adjacent tissues. HNSCC: Head and neck squamous cell carcinoma, NPC Nasopharyngeal carcinoma, cSCC Cutaneous squamous cell carcinoma. Data are presented as mean ± s.e.m. ns: not significant ( P > 0.05). Source data are provided as a Source Data file.
    Human Anti Cd86, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+cd86/pm41162642-335-0-2?v=Cell+Signaling+Technology+Inc
    Average 96 stars, based on 1 article reviews
    human anti cd86 - by Bioz Stars, 2026-08
    96/100 stars
    		  Buy from Supplier
    rabbit anti human cd86  (Wanleibio)
    86
    Wanleibio rabbit anti human cd86
    A Schematic overview of the experimental strategy (Created in BioRender). Pan, W. (2025) https://biorender.com/udc23v6 . Surgical tumor specimens and paired normal adjacent tissues (NATs) were collected from 23 treatment-naive PSCC patients (Tumor, n = 23; NATs, n = 5) and processed for single-cell RNA sequencing and transcriptomic analysis. B UMAP plot of 202,424 cells from the discovery cohort, showing cell components in PSCC, color-coded by cell type (left), patients (middle) and tissue (right). C Normalized expression of marker genes used to define four major cell types as shown in ( B ). D Relative abundance of four major cell types in tumor tissues ( n = 23) or NATs ( n = 5) from each PSCC patient, excluding PN4. E Bar graph comparing the relative proportions of the four cell types between tumor and NATs. P- values were assessed using a two-sided unpaired Student’s t test (Immu: **** P < 0.0001, Endo: **** P < 0.0001, CAFs: ** P = 0.002). F Multiplex immunofluorescence staining depicting the presence of four major cell components (CD45 + immune cells, KRT5 + epithelial cells, CD31 + endothelial cells, and VIM + fibroblasts) in tumor and NATs of PSCC. Representative images include DAPI + nuclei for reference. Scale: 50 μm. G Multiplex immunofluorescence depicting the distribution of five major immune subpopulations (CD4 + T cells, CD8 + T cells, <t>CD86</t> + antigen-presenting cells, CD20 + B cells, CD56 + natural killer cells) in tumor and NATs of PSCC. Representative images include DAPI + nuclei for reference. Scale: 200 μm. H Quantification of CD4 + , CD8 + , CD86 + , CD20 + and CD56 + cells per area in paired tumor and NATs regions in PSCC ( n = 6). P- values were assessed using a two-sided paired Student’s t test (CD4 + : **** P < 0.0001, CD8 + : *** P = 0.0002, CD86 + : *** P = 0.0002, CD20 + : **** P < 0.0001, CD56 + : ** P = 0.0053). I UMAP plot comparing the TME composition in PSCC with other squamous cell carcinomas, including HNSCC ( GSE103322 ), NPC ( GSE150430 ), and cSCC ( GSE144236 ). J Bar graph showing the relative proportions of the four cell types across these tumor types. PN PSCC adjacent normal tissue, PT PSCC tumor tissue. NATs Normal adjacent tissues. HNSCC: Head and neck squamous cell carcinoma, NPC Nasopharyngeal carcinoma, cSCC Cutaneous squamous cell carcinoma. Data are presented as mean ± s.e.m. ns: not significant ( P > 0.05). Source data are provided as a Source Data file.
    Rabbit Anti Human Cd86, supplied by Wanleibio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+cd86/10__1016_slash_j__bioactmat__2025__09__008-154-45-49?v=Wanleibio
    Average 86 stars, based on 1 article reviews
    rabbit anti human cd86 - by Bioz Stars, 2026-08
    86/100 stars
    		  Buy from Supplier
    anti cd86 human cell signaling technology 76755 if  (Cell Signaling Technology Inc)
    94
    Cell Signaling Technology Inc anti cd86 human cell signaling technology 76755 if
    A Schematic overview of the experimental strategy (Created in BioRender). Pan, W. (2025) https://biorender.com/udc23v6 . Surgical tumor specimens and paired normal adjacent tissues (NATs) were collected from 23 treatment-naive PSCC patients (Tumor, n = 23; NATs, n = 5) and processed for single-cell RNA sequencing and transcriptomic analysis. B UMAP plot of 202,424 cells from the discovery cohort, showing cell components in PSCC, color-coded by cell type (left), patients (middle) and tissue (right). C Normalized expression of marker genes used to define four major cell types as shown in ( B ). D Relative abundance of four major cell types in tumor tissues ( n = 23) or NATs ( n = 5) from each PSCC patient, excluding PN4. E Bar graph comparing the relative proportions of the four cell types between tumor and NATs. P- values were assessed using a two-sided unpaired Student’s t test (Immu: **** P < 0.0001, Endo: **** P < 0.0001, CAFs: ** P = 0.002). F Multiplex immunofluorescence staining depicting the presence of four major cell components (CD45 + immune cells, KRT5 + epithelial cells, CD31 + endothelial cells, and VIM + fibroblasts) in tumor and NATs of PSCC. Representative images include DAPI + nuclei for reference. Scale: 50 μm. G Multiplex immunofluorescence depicting the distribution of five major immune subpopulations (CD4 + T cells, CD8 + T cells, <t>CD86</t> + antigen-presenting cells, CD20 + B cells, CD56 + natural killer cells) in tumor and NATs of PSCC. Representative images include DAPI + nuclei for reference. Scale: 200 μm. H Quantification of CD4 + , CD8 + , CD86 + , CD20 + and CD56 + cells per area in paired tumor and NATs regions in PSCC ( n = 6). P- values were assessed using a two-sided paired Student’s t test (CD4 + : **** P < 0.0001, CD8 + : *** P = 0.0002, CD86 + : *** P = 0.0002, CD20 + : **** P < 0.0001, CD56 + : ** P = 0.0053). I UMAP plot comparing the TME composition in PSCC with other squamous cell carcinomas, including HNSCC ( GSE103322 ), NPC ( GSE150430 ), and cSCC ( GSE144236 ). J Bar graph showing the relative proportions of the four cell types across these tumor types. PN PSCC adjacent normal tissue, PT PSCC tumor tissue. NATs Normal adjacent tissues. HNSCC: Head and neck squamous cell carcinoma, NPC Nasopharyngeal carcinoma, cSCC Cutaneous squamous cell carcinoma. Data are presented as mean ± s.e.m. ns: not significant ( P > 0.05). Source data are provided as a Source Data file.
    Anti Cd86 Human Cell Signaling Technology 76755 If, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+cd86/pmc12159176__42003_2025_8147_MOESM1_ESM-2-29-31?v=Cell+Signaling+Technology+Inc
    Average 94 stars, based on 1 article reviews
    anti cd86 human cell signaling technology 76755 if - by Bioz Stars, 2026-08
    94/100 stars
    		  Buy from Supplier
    rabbit anti human cd86 antibody  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc rabbit anti human cd86 antibody
    A Schematic overview of the experimental strategy (Created in BioRender). Pan, W. (2025) https://biorender.com/udc23v6 . Surgical tumor specimens and paired normal adjacent tissues (NATs) were collected from 23 treatment-naive PSCC patients (Tumor, n = 23; NATs, n = 5) and processed for single-cell RNA sequencing and transcriptomic analysis. B UMAP plot of 202,424 cells from the discovery cohort, showing cell components in PSCC, color-coded by cell type (left), patients (middle) and tissue (right). C Normalized expression of marker genes used to define four major cell types as shown in ( B ). D Relative abundance of four major cell types in tumor tissues ( n = 23) or NATs ( n = 5) from each PSCC patient, excluding PN4. E Bar graph comparing the relative proportions of the four cell types between tumor and NATs. P- values were assessed using a two-sided unpaired Student’s t test (Immu: **** P < 0.0001, Endo: **** P < 0.0001, CAFs: ** P = 0.002). F Multiplex immunofluorescence staining depicting the presence of four major cell components (CD45 + immune cells, KRT5 + epithelial cells, CD31 + endothelial cells, and VIM + fibroblasts) in tumor and NATs of PSCC. Representative images include DAPI + nuclei for reference. Scale: 50 μm. G Multiplex immunofluorescence depicting the distribution of five major immune subpopulations (CD4 + T cells, CD8 + T cells, <t>CD86</t> + antigen-presenting cells, CD20 + B cells, CD56 + natural killer cells) in tumor and NATs of PSCC. Representative images include DAPI + nuclei for reference. Scale: 200 μm. H Quantification of CD4 + , CD8 + , CD86 + , CD20 + and CD56 + cells per area in paired tumor and NATs regions in PSCC ( n = 6). P- values were assessed using a two-sided paired Student’s t test (CD4 + : **** P < 0.0001, CD8 + : *** P = 0.0002, CD86 + : *** P = 0.0002, CD20 + : **** P < 0.0001, CD56 + : ** P = 0.0053). I UMAP plot comparing the TME composition in PSCC with other squamous cell carcinomas, including HNSCC ( GSE103322 ), NPC ( GSE150430 ), and cSCC ( GSE144236 ). J Bar graph showing the relative proportions of the four cell types across these tumor types. PN PSCC adjacent normal tissue, PT PSCC tumor tissue. NATs Normal adjacent tissues. HNSCC: Head and neck squamous cell carcinoma, NPC Nasopharyngeal carcinoma, cSCC Cutaneous squamous cell carcinoma. Data are presented as mean ± s.e.m. ns: not significant ( P > 0.05). Source data are provided as a Source Data file.
    Rabbit Anti Human Cd86 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+cd86/pm40304130-268-5-54?v=Cell+Signaling+Technology+Inc
    Average 96 stars, based on 1 article reviews
    rabbit anti human cd86 antibody - by Bioz Stars, 2026-08
    96/100 stars
    		  Buy from Supplier
    rabbit anti human cd86 antibody  (Proteintech)
    96
    Proteintech rabbit anti human cd86 antibody
    A Schematic overview of the experimental strategy (Created in BioRender). Pan, W. (2025) https://biorender.com/udc23v6 . Surgical tumor specimens and paired normal adjacent tissues (NATs) were collected from 23 treatment-naive PSCC patients (Tumor, n = 23; NATs, n = 5) and processed for single-cell RNA sequencing and transcriptomic analysis. B UMAP plot of 202,424 cells from the discovery cohort, showing cell components in PSCC, color-coded by cell type (left), patients (middle) and tissue (right). C Normalized expression of marker genes used to define four major cell types as shown in ( B ). D Relative abundance of four major cell types in tumor tissues ( n = 23) or NATs ( n = 5) from each PSCC patient, excluding PN4. E Bar graph comparing the relative proportions of the four cell types between tumor and NATs. P- values were assessed using a two-sided unpaired Student’s t test (Immu: **** P < 0.0001, Endo: **** P < 0.0001, CAFs: ** P = 0.002). F Multiplex immunofluorescence staining depicting the presence of four major cell components (CD45 + immune cells, KRT5 + epithelial cells, CD31 + endothelial cells, and VIM + fibroblasts) in tumor and NATs of PSCC. Representative images include DAPI + nuclei for reference. Scale: 50 μm. G Multiplex immunofluorescence depicting the distribution of five major immune subpopulations (CD4 + T cells, CD8 + T cells, <t>CD86</t> + antigen-presenting cells, CD20 + B cells, CD56 + natural killer cells) in tumor and NATs of PSCC. Representative images include DAPI + nuclei for reference. Scale: 200 μm. H Quantification of CD4 + , CD8 + , CD86 + , CD20 + and CD56 + cells per area in paired tumor and NATs regions in PSCC ( n = 6). P- values were assessed using a two-sided paired Student’s t test (CD4 + : **** P < 0.0001, CD8 + : *** P = 0.0002, CD86 + : *** P = 0.0002, CD20 + : **** P < 0.0001, CD56 + : ** P = 0.0053). I UMAP plot comparing the TME composition in PSCC with other squamous cell carcinomas, including HNSCC ( GSE103322 ), NPC ( GSE150430 ), and cSCC ( GSE144236 ). J Bar graph showing the relative proportions of the four cell types across these tumor types. PN PSCC adjacent normal tissue, PT PSCC tumor tissue. NATs Normal adjacent tissues. HNSCC: Head and neck squamous cell carcinoma, NPC Nasopharyngeal carcinoma, cSCC Cutaneous squamous cell carcinoma. Data are presented as mean ± s.e.m. ns: not significant ( P > 0.05). Source data are provided as a Source Data file.
    Rabbit Anti Human Cd86 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+cd86/pm39939274-58-6-11?v=Proteintech
    Average 96 stars, based on 1 article reviews
    rabbit anti human cd86 antibody - by Bioz Stars, 2026-08
    96/100 stars
    		  Buy from Supplier
    anti human cd86 antibody  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc anti human cd86 antibody
    A Schematic overview of the experimental strategy (Created in BioRender). Pan, W. (2025) https://biorender.com/udc23v6 . Surgical tumor specimens and paired normal adjacent tissues (NATs) were collected from 23 treatment-naive PSCC patients (Tumor, n = 23; NATs, n = 5) and processed for single-cell RNA sequencing and transcriptomic analysis. B UMAP plot of 202,424 cells from the discovery cohort, showing cell components in PSCC, color-coded by cell type (left), patients (middle) and tissue (right). C Normalized expression of marker genes used to define four major cell types as shown in ( B ). D Relative abundance of four major cell types in tumor tissues ( n = 23) or NATs ( n = 5) from each PSCC patient, excluding PN4. E Bar graph comparing the relative proportions of the four cell types between tumor and NATs. P- values were assessed using a two-sided unpaired Student’s t test (Immu: **** P < 0.0001, Endo: **** P < 0.0001, CAFs: ** P = 0.002). F Multiplex immunofluorescence staining depicting the presence of four major cell components (CD45 + immune cells, KRT5 + epithelial cells, CD31 + endothelial cells, and VIM + fibroblasts) in tumor and NATs of PSCC. Representative images include DAPI + nuclei for reference. Scale: 50 μm. G Multiplex immunofluorescence depicting the distribution of five major immune subpopulations (CD4 + T cells, CD8 + T cells, <t>CD86</t> + antigen-presenting cells, CD20 + B cells, CD56 + natural killer cells) in tumor and NATs of PSCC. Representative images include DAPI + nuclei for reference. Scale: 200 μm. H Quantification of CD4 + , CD8 + , CD86 + , CD20 + and CD56 + cells per area in paired tumor and NATs regions in PSCC ( n = 6). P- values were assessed using a two-sided paired Student’s t test (CD4 + : **** P < 0.0001, CD8 + : *** P = 0.0002, CD86 + : *** P = 0.0002, CD20 + : **** P < 0.0001, CD56 + : ** P = 0.0053). I UMAP plot comparing the TME composition in PSCC with other squamous cell carcinomas, including HNSCC ( GSE103322 ), NPC ( GSE150430 ), and cSCC ( GSE144236 ). J Bar graph showing the relative proportions of the four cell types across these tumor types. PN PSCC adjacent normal tissue, PT PSCC tumor tissue. NATs Normal adjacent tissues. HNSCC: Head and neck squamous cell carcinoma, NPC Nasopharyngeal carcinoma, cSCC Cutaneous squamous cell carcinoma. Data are presented as mean ± s.e.m. ns: not significant ( P > 0.05). Source data are provided as a Source Data file.
    Anti Human Cd86 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+cd86/pmc12002222__BLOOD_BLD___2023___022197R1___mmc1-220-8-11?v=Cell+Signaling+Technology+Inc
    Average 96 stars, based on 1 article reviews
    anti human cd86 antibody - by Bioz Stars, 2026-08
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Expression of CD68, CD86 and CD206 in decidual tissues of normal pregnancy women and patients with PE. ( A ) The expression levels of CD68, CD86 and CD206 in decidual tissues were assessed by immunohistochemistry (×200). ( B ) Semiquantitative analysis of ( A ). ( C ) The relative protein levels of CD86 and CD206 were detected via Western blotting. GAPDH was used as an endogenous control. ( D ) Semiquantitative analysis of ( C ). ( E ) The relative mRNA levels of TNF-α and IL-6 were detected via q-PCR. Student’s t -test was used for the statistical analysis, and *p<0.05; **p < 0.01.

    Journal: International Journal of Women's Health

    Article Title: Altered Expression of CXCL16/CXCR6 and Its Correlation with Decidual Macrophage Polarization in Preeclampsia

    doi: 10.2147/IJWH.S567928

    Figure Lengend Snippet: Expression of CD68, CD86 and CD206 in decidual tissues of normal pregnancy women and patients with PE. ( A ) The expression levels of CD68, CD86 and CD206 in decidual tissues were assessed by immunohistochemistry (×200). ( B ) Semiquantitative analysis of ( A ). ( C ) The relative protein levels of CD86 and CD206 were detected via Western blotting. GAPDH was used as an endogenous control. ( D ) Semiquantitative analysis of ( C ). ( E ) The relative mRNA levels of TNF-α and IL-6 were detected via q-PCR. Student’s t -test was used for the statistical analysis, and *p<0.05; **p < 0.01.

    Article Snippet: Membranes were blocked for 15 min at room temperature with rapid blocking buffer (NCM Biotech, Suzhou, China), and subsequently incubated with the following primary antibodies: mouse anti-human GAPDH (1:100000, Proteintech, Wuhan, China), rabbit anti-human CD86 (1:1000, Proteintech, Wuhan, China), rabbit anti-human CD206 (1:1000, Proteintech, Wuhan, China), rabbit anti-human CXCL16 (1:1000, Thermo Fisher Scientific, Shanghai, China), and rabbit anti-human CXCR6 (1:1000, Proteintech, Wuhan, China).

    Techniques: Expressing, Immunohistochemistry, Western Blot, Control

    Immunofluorescence staining for M1 and M2 in normal pregnancy and patients with PE. CD68 is the maker for pan-macrophages. CD80 and CD86 are markers for M1, CD163 and CD206 are markers for M2 macrophages. Scale bars: 100 µm. ( A and B ) The CD68 + CD80 + and CD68 + CD86 + cells in decidual tissues were detected by immunofluorescence double staining. ( C and D ) The CD68 + CD163 + and CD68 + CD206 + cells in decidual tissues were detected by immunofluorescence double staining.

    Journal: International Journal of Women's Health

    Article Title: Altered Expression of CXCL16/CXCR6 and Its Correlation with Decidual Macrophage Polarization in Preeclampsia

    doi: 10.2147/IJWH.S567928

    Figure Lengend Snippet: Immunofluorescence staining for M1 and M2 in normal pregnancy and patients with PE. CD68 is the maker for pan-macrophages. CD80 and CD86 are markers for M1, CD163 and CD206 are markers for M2 macrophages. Scale bars: 100 µm. ( A and B ) The CD68 + CD80 + and CD68 + CD86 + cells in decidual tissues were detected by immunofluorescence double staining. ( C and D ) The CD68 + CD163 + and CD68 + CD206 + cells in decidual tissues were detected by immunofluorescence double staining.

    Article Snippet: Membranes were blocked for 15 min at room temperature with rapid blocking buffer (NCM Biotech, Suzhou, China), and subsequently incubated with the following primary antibodies: mouse anti-human GAPDH (1:100000, Proteintech, Wuhan, China), rabbit anti-human CD86 (1:1000, Proteintech, Wuhan, China), rabbit anti-human CD206 (1:1000, Proteintech, Wuhan, China), rabbit anti-human CXCL16 (1:1000, Thermo Fisher Scientific, Shanghai, China), and rabbit anti-human CXCR6 (1:1000, Proteintech, Wuhan, China).

    Techniques: Immunofluorescence, Staining, Double Staining

    Decidual macrophages polarization of normal pregnant women and patients with PE. ( A ) Flow cytometric analysis to determine the proportion of M1 (CD68 + CD80 + or CD68 + CD86 + ) and M2 (CD68 + CD163 + or CD68 + CD206 + ) in normal pregnancy and patients with PE. ( B ) Semiquantitative analysis of ( A ). Student’s t -test was used for the statistical analysis, and **p<0.01; ***p < 0.001; ****p < 0.0001.

    Journal: International Journal of Women's Health

    Article Title: Altered Expression of CXCL16/CXCR6 and Its Correlation with Decidual Macrophage Polarization in Preeclampsia

    doi: 10.2147/IJWH.S567928

    Figure Lengend Snippet: Decidual macrophages polarization of normal pregnant women and patients with PE. ( A ) Flow cytometric analysis to determine the proportion of M1 (CD68 + CD80 + or CD68 + CD86 + ) and M2 (CD68 + CD163 + or CD68 + CD206 + ) in normal pregnancy and patients with PE. ( B ) Semiquantitative analysis of ( A ). Student’s t -test was used for the statistical analysis, and **p<0.01; ***p < 0.001; ****p < 0.0001.

    Article Snippet: Membranes were blocked for 15 min at room temperature with rapid blocking buffer (NCM Biotech, Suzhou, China), and subsequently incubated with the following primary antibodies: mouse anti-human GAPDH (1:100000, Proteintech, Wuhan, China), rabbit anti-human CD86 (1:1000, Proteintech, Wuhan, China), rabbit anti-human CD206 (1:1000, Proteintech, Wuhan, China), rabbit anti-human CXCL16 (1:1000, Thermo Fisher Scientific, Shanghai, China), and rabbit anti-human CXCR6 (1:1000, Proteintech, Wuhan, China).

    Techniques:

    A Schematic overview of the experimental strategy (Created in BioRender). Pan, W. (2025) https://biorender.com/udc23v6 . Surgical tumor specimens and paired normal adjacent tissues (NATs) were collected from 23 treatment-naive PSCC patients (Tumor, n = 23; NATs, n = 5) and processed for single-cell RNA sequencing and transcriptomic analysis. B UMAP plot of 202,424 cells from the discovery cohort, showing cell components in PSCC, color-coded by cell type (left), patients (middle) and tissue (right). C Normalized expression of marker genes used to define four major cell types as shown in ( B ). D Relative abundance of four major cell types in tumor tissues ( n = 23) or NATs ( n = 5) from each PSCC patient, excluding PN4. E Bar graph comparing the relative proportions of the four cell types between tumor and NATs. P- values were assessed using a two-sided unpaired Student’s t test (Immu: **** P < 0.0001, Endo: **** P < 0.0001, CAFs: ** P = 0.002). F Multiplex immunofluorescence staining depicting the presence of four major cell components (CD45 + immune cells, KRT5 + epithelial cells, CD31 + endothelial cells, and VIM + fibroblasts) in tumor and NATs of PSCC. Representative images include DAPI + nuclei for reference. Scale: 50 μm. G Multiplex immunofluorescence depicting the distribution of five major immune subpopulations (CD4 + T cells, CD8 + T cells, CD86 + antigen-presenting cells, CD20 + B cells, CD56 + natural killer cells) in tumor and NATs of PSCC. Representative images include DAPI + nuclei for reference. Scale: 200 μm. H Quantification of CD4 + , CD8 + , CD86 + , CD20 + and CD56 + cells per area in paired tumor and NATs regions in PSCC ( n = 6). P- values were assessed using a two-sided paired Student’s t test (CD4 + : **** P < 0.0001, CD8 + : *** P = 0.0002, CD86 + : *** P = 0.0002, CD20 + : **** P < 0.0001, CD56 + : ** P = 0.0053). I UMAP plot comparing the TME composition in PSCC with other squamous cell carcinomas, including HNSCC ( GSE103322 ), NPC ( GSE150430 ), and cSCC ( GSE144236 ). J Bar graph showing the relative proportions of the four cell types across these tumor types. PN PSCC adjacent normal tissue, PT PSCC tumor tissue. NATs Normal adjacent tissues. HNSCC: Head and neck squamous cell carcinoma, NPC Nasopharyngeal carcinoma, cSCC Cutaneous squamous cell carcinoma. Data are presented as mean ± s.e.m. ns: not significant ( P > 0.05). Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: A B cell-IgA-epithelial axis enhances antitumor immunity and improves outcome in HPV-associated penile squamous cell carcinoma

    doi: 10.1038/s41467-025-67356-6

    Figure Lengend Snippet: A Schematic overview of the experimental strategy (Created in BioRender). Pan, W. (2025) https://biorender.com/udc23v6 . Surgical tumor specimens and paired normal adjacent tissues (NATs) were collected from 23 treatment-naive PSCC patients (Tumor, n = 23; NATs, n = 5) and processed for single-cell RNA sequencing and transcriptomic analysis. B UMAP plot of 202,424 cells from the discovery cohort, showing cell components in PSCC, color-coded by cell type (left), patients (middle) and tissue (right). C Normalized expression of marker genes used to define four major cell types as shown in ( B ). D Relative abundance of four major cell types in tumor tissues ( n = 23) or NATs ( n = 5) from each PSCC patient, excluding PN4. E Bar graph comparing the relative proportions of the four cell types between tumor and NATs. P- values were assessed using a two-sided unpaired Student’s t test (Immu: **** P < 0.0001, Endo: **** P < 0.0001, CAFs: ** P = 0.002). F Multiplex immunofluorescence staining depicting the presence of four major cell components (CD45 + immune cells, KRT5 + epithelial cells, CD31 + endothelial cells, and VIM + fibroblasts) in tumor and NATs of PSCC. Representative images include DAPI + nuclei for reference. Scale: 50 μm. G Multiplex immunofluorescence depicting the distribution of five major immune subpopulations (CD4 + T cells, CD8 + T cells, CD86 + antigen-presenting cells, CD20 + B cells, CD56 + natural killer cells) in tumor and NATs of PSCC. Representative images include DAPI + nuclei for reference. Scale: 200 μm. H Quantification of CD4 + , CD8 + , CD86 + , CD20 + and CD56 + cells per area in paired tumor and NATs regions in PSCC ( n = 6). P- values were assessed using a two-sided paired Student’s t test (CD4 + : **** P < 0.0001, CD8 + : *** P = 0.0002, CD86 + : *** P = 0.0002, CD20 + : **** P < 0.0001, CD56 + : ** P = 0.0053). I UMAP plot comparing the TME composition in PSCC with other squamous cell carcinomas, including HNSCC ( GSE103322 ), NPC ( GSE150430 ), and cSCC ( GSE144236 ). J Bar graph showing the relative proportions of the four cell types across these tumor types. PN PSCC adjacent normal tissue, PT PSCC tumor tissue. NATs Normal adjacent tissues. HNSCC: Head and neck squamous cell carcinoma, NPC Nasopharyngeal carcinoma, cSCC Cutaneous squamous cell carcinoma. Data are presented as mean ± s.e.m. ns: not significant ( P > 0.05). Source data are provided as a Source Data file.

    Article Snippet: Primary antibodies were mouse-anti-human KRT5 (ZsBio, TA800727), mouse-anti-human CD45 (ZsBio, ZM-0183), mouse-anti-human CD31 (ZsBio, ZM-0044), rabbit-anti-human VIM (Abram, ab92547), mouse-anti-human CD3 (Servicebio, GB12014), mouse anti-human CD4 (ZsBio, ZM-0418), mouse-anti-human CD8 (Servicebio, GB12068), mouse-anti-human CD20 (Servicebio, GB14030), mouse-anti-human CD38 (Servicebio, GB114831 ), mouse-anti-human CD56 (ZsBio, ZM-0057), rabbit-anti-human CD86 (CST, 91882S), rabbit-anti-human IgA (Abcam, ab124716), rabbit-anti-human PIGR (Abcam, ab96196).

    Techniques: RNA Sequencing, Expressing, Marker, Multiplex Assay, Immunofluorescence, Staining